Date of Award
5-26-2015
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Applied Science
First Advisor
Mariya Khodakovskaya
Abstract
This thesis project involves increasing the tolerance to abiotic stresses in rice through silencing the OsHP gene, a homolog of Arabidopsis AtClb, which has been identified as a negative regulator of response to abiotic stresses. A fragment of the OsHP gene was cloned into a Gateway entry vector pENTR/D-TOPO, and a silencing construct was created with the Gateway destination vector pANDA through an LR clonase reaction. Embryogenic callus tissue was generated from seed of the rice cultivars `Nipponbare' and `LaGrue'. Biolistic and Agrobacterium-mediated transformation procedures were used to transfer the pANDA_OsHP silencing construct into the rice callus tissue. Transformed calli were able to generate morphogenic structures on selective regenerative medium. Morphogenic callus tissue will be subjects for regeneration of transgenic rice plants with silenced OsHP gene and future stress experiments.
Recommended Citation
Boyett, Virginia Ann, "Silencing of the OsHP Gene for Increasing Abiotic Stress Tolerance in Rice" (2015). Theses and Dissertations. 578.
https://research.ualr.edu/etd/578
