Date of Award
11-28-2016
Document Type
Thesis
Degree Name
Master of Biological Science (MBioSci)
Department
Biology
First Advisor
Stephen Grace
Abstract
Tomato (Solanum lycopersicon) is the leading vegetable crop worldwide and an important part of a healthy human diet. In this study we used Next-Generation Sequencing, metabolomics and gene ontologies to study the effects of high light stress on the photomorphogenic hp-2dg tomato mutant. Wild-type (cv. ‘Manapal’) and mutant plants were grown for six weeks under low light and transferred to high light for one week. Illumina-based RNA Sequencing was performed and analysis of differential gene expression identified over 650 transcripts that were strongly regulated by light intensity (FC>4, p2,p<0,01). HPLC analysis of tomato leaves showed differential accumulation of phenylpropanoid metabolites such as chlorogenic acid, rutin, and caffeoylglucaric acid which was strongly dependent on genotype and light condition. Our findings show insightful differences between the wildtype and the photomorphogenic mutant as well as novel genes involved in high light adaptation in plants.
Recommended Citation
Radke, Jordyn, "Linking Transcriptional and Physiological Responses to High Light Stress in a Wild-Type and a Photomorphogenic Tomato Mutant" (2016). Theses and Dissertations. 720.
https://research.ualr.edu/etd/720
